Figure 4.

Effect of sst2 expression on in vitro invasivenes of pancreatic cancer cells. Cells were plated in chamber containing a Matrigel-coated filter at its base. The chambers were immersed in RPMI medium 1640 with 1% of BSA for 30 min at 37°C. After 48-h incubation, the noninvasive cells, which remain on the topside of the filter, were removed, and the invasive cells, which attach themselves to the underside of the filter, were stained with 0.2% crystal violet after fixing with 3% paraformaldehyde. The figure represents a photograph at a low magnification (×40) of filters with stained cells (A, PC-1.0 cells; B, PC-1.0/sst2 cells) and is representative of four separate experiments.