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. 2000 Jul 25;97(16):9186–9190. doi: 10.1073/pnas.160254997

Figure 3.

Figure 3

Survival of ESMCs in vivo. (A) Mast cell-deficient WBB6F1-KitW/KitW-v mice (n = 6–15/group, 8–16 wk old) were injected i.p. with 2 × 106 SEK1 +/+ or SEK1 −/− ESMCs, or BMCMCs of WBB6F1-+/+ (Kit+/+) origin. At 3–4 or 8 wk after injections, peritoneal lavage was performed, and the percentage of mast cells present in the lavage was evaluated by Kimura staining. The percentage of native peritoneal mast cells (PMCs) in WBB6F1-+/+ (Kit+/+) mice was counted as the positive control (black bar). *, P < 0.05 as compared with values for mice at 3 wk after injection of SEK1 −/− ESMCs or BMCMCs. (B and C) Flow cytometry analysis of c-kit and FcɛRI expression on the ESMCs recovered from the peritoneal cavities of WBB6F1-KitW/KitW-v mice 8 wk after reconstitution (B) or on PMCs recovered from Kit+/+ mice (C); mast cells are indicated by the oval.