Figure 5.

Conformation of p53 in RKO and HCT 116 cells treated with electrophilic PG. Immunochemical determination of mutant conformation of p53 from RKO cells (Upper) and HCT 116 cells (Lower) incubated with DMSO (Vehicle), 20–60 μM PGA₁ or PGA₂, along with controls of cells transfected with mutant (mt) and wild-type (wt) p53 constructs and 60 μM PGB₁. Lanes represent the relative proportions of mutant conformation p53 immunoprecipitated (IP) from the total cell lysate by using Pab240 and detected by immunoblotting (IB) with FL-393.