Figure 6.
Impairment of p53-mediated apoptosis in RKO and HCT 116 cells treated with electrophilic PG. Caspase-3 activity increased in RKO cells (Upper) and HCT 116 cells (Lower) incubated with 50 μM etoposide (Etop; ●) for 0–24 h. In cells treated with 50 μM etoposide plus 20 μM PGA1 (▴) or plus 20 μM PGA2 (▾), the rate and maximal extent of caspase-3 activation fell compared with etoposide alone (●). The time course for caspase-3 activity was similar among cells treated with DMSO vehicle control, 20 μM PGA1 (▵), or 20 μM PGA2 (▿) alone.