Figure 2.

Comparison of the staining patterns of Fab ANA15 and the donor serum (patient with Felty's syndrome) on ethanol-fixed HEp-2 cells and neutrophils with indirect IF. For HEp-2 cells, distinctly different staining for the two reagents was observed, whereas similar staining patterns were observed for neutrophils. Fab ANA15 stained neutrophils (arrows) but not neighboring lymphocytes (arrowheads). No staining was observed with a control Fab against HIV-1 gp120 or secondary antibody alone (not shown).