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. 2000 Jul 25;97(16):9240–9245. doi: 10.1073/pnas.160070497

Figure 1.

Figure 1

Genes necessary for functional pili. The type 1 pilus gene cluster and gene products necessary for the expression and assembly of type 1 pili are shown (reviewed in ref. 38). See Introduction for a description of the gene products represented as A–H in the schematic depiction of the fim operon shown at the top. The bar graph indicates the ability of various combinations of type 1 genes to encode for production of functional type 1 pili as assessed by the hemagglutination (HA) of guinea pig red blood cells (39). The HA titer produced by ORN103/pETS9 (1:256) was identical to that of a type 1 operon under the control of its wild-type promoter or an NU14 clinical isolate. In a two-plasmid system, the HA titer matched that of the wild type, whereas with a nonfunctional mutant fimD (pETS7A743D) no HA occurred (see Materials and Methods for information regarding mutant).