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Gel mobility shifts of a 32P-labeled comK promoter fragment. (A) Incubation with increasing concentrations of DegU. Concentrations are given in μM. (B) Effect of DegU (D) on ComK (K)-induced retardation. Protein concentrations used were 0.15 μM and 0.22 μM for DegU and ComK, respectively. The left lane (0) contained no protein.
