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Effect of DNA binding agents on the binding of ComK to the comK promoter. A 32P-labeled comK promoter fragment was incubated with 0.4 μM ComK (K) in the absence (−) or presence of 6 μM actinomycin D (A) or 6 μM ethidium bromide (E). No substantial retardation of the comK promoter fragment was observed when only actinomycin D or ethidium bromide was present (not shown).
