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. 2000 Jul 25;97(16):9258–9263. doi: 10.1073/pnas.160257897

Figure 1.

Figure 1

Disruption of the LPG1 locus. (A) Genomic organization of the L. major LPG1 locus and the planned replacements LPG1∷PAC and LPG1∷HYG. S, X, and B indicate SmaI, XhoI, and BamHI restriction sites, respectively; the targeting fragments contained the regions marked by the BamHI sites. The sizes of fragments (in kb) expected following digestion with SmaI and hybridization with the probe denoted by the black bar are shown above each map. (B) Southern blot analysis of WT (+/+, lane 1), heterozygous (+/−, lane 2, clone H2), and homozygous mutants (−/−, lane 3, clone A3) DNAs.