Figure 2.

lpg1⁻ L. major lack LPG. (A) LPG was metabolically labeled with [³H]mannose, purified, incorporation determined by liquid scintillation counting, and the levels expressed relative to WT. Only background levels were detected in lpg1⁻ cells. (B) Purified LPGs were subjected to Western blot analysis with anti-PG antibody WIC 79.3. No reactive material was detected in the lpg1⁻ cells. (C) Flow cytometry of live Leishmania labeled with fluorescein-conjugated ricin agglutinin. The control shows unlabeled WT parasites.