Figure 5.

lpg1⁻ cells maintain Gal_f-containing GIPLs. (A) WT and lpg1⁻ GIPLs, metabolically labeled with [³H]galactose were separated by paper chromatographic procedures. The mobility of unlabeled standards are indicated; Gal, galactose; Glc, glucose; Gal_f, galactofuranose; Man, mannose. (B) Total GIPL analysis. Purified GIPLs were delipidated, fluorophore-labeled, separated by the GLYKO-FACE system (GLYKO), and visualized by UV fluorescence. The arrow indicates the mobility of the derivative corresponding to LPG precursor Glc-P-Man₂-GlcN-PI. G2–G5 represent oligomeric derivatives of glucose.