Figure 5.

The ability of the GRE5 promoter to be induced in vivo by exogenous corticosterone and KA-induced excitotoxic seizures was examined after injection of vGRE(L)lacZ into rat hippocampus. (A) In injected rats that received no corticosterone, no lacZ expression occurred after X-gal staining of sections taken from rats 24 h postsurgery. (B) Injected rats that received corticosterone showed a number of X-gal-positive cells surrounding the injection site in dentate gyrus. (C) Higher magnification view of X-gal-positive cells shown in B. (D) Rats that received KA infusion into the dentate gyrus exhibited high numbers of X-gal-positive cells surrounding the injection site. (Scale bars: A and B = 500 μm; C and D = 200 μm.)