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. 1997 Jul;63(7):2844–2849. doi: 10.1128/aem.63.7.2844-2849.1997

A spontaneous change in the intracellular cyclic AMP level in Aspergillus niger is influenced by the sucrose concentration in the medium and by light.

M Gradisnik-Grapulin 1, M Legisa 1
PMCID: PMC168580  PMID: 9212431

Abstract

A spontaneous rise in intracellular cyclic AMP (cAMP) levels was observed in the early stages of Aspergillus niger growth under conditions yielding large amounts of citric acid. The amount of cAMP formed was found to depend on the initial concentration of sucrose in the medium. Under higher-sucrose conditions, the cAMP peak appeared earlier and was higher, while in lower-sucrose media a flattened peak was observed later in fermentation. Since in media with higher concentrations of sucrose intracellular citric acid starts to accumulate earlier and more rapidly, cAMP synthesis may be triggered by intracellular acidification, which is caused by the dissociation of citric acid. No spontaneous increase in cAMP concentrations could be detected when the cells were grown in continuously illuminated cultures, suggesting that A. niger phosphodiesterase (PDE) is photoregulated. More evidence for the activation of PDE by light was obtained from morphological studies under light and dark conditions in the presence of cAMP or N6,O2'-dibutyryl cAMP, and this idea was additionally supported by experiments in which PDE inhibitors were tested.

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Selected References

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  1. Beavo J. A. Multiple isozymes of cyclic nucleotide phosphodiesterase. Adv Second Messenger Phosphoprotein Res. 1988;22:1–38. [PubMed] [Google Scholar]
  2. Cho T., Hamatake H., Kaminishi H., Hagihara Y., Watanabe K. The relationship between cyclic adenosine 3',5'-monophosphate and morphology in exponential phase Candida albicans. J Med Vet Mycol. 1992;30(1):35–42. [PubMed] [Google Scholar]
  3. Hurley J. B. Molecular properties of the cGMP cascade of vertebrate photoreceptors. Annu Rev Physiol. 1987;49:793–812. doi: 10.1146/annurev.ph.49.030187.004045. [DOI] [PubMed] [Google Scholar]
  4. Larsen A. D., Sypherd P. S. Cyclic adenosine 3',5'-monophosphate and morphogenesis in Mucor racemosus. J Bacteriol. 1974 Feb;117(2):432–438. doi: 10.1128/jb.117.2.432-438.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Legisa M., Bencina M. Evidence for the activation of 6-phosphofructo-1-kinase by cAMP-dependent protein kinase in Aspergillus niger. FEMS Microbiol Lett. 1994 May 15;118(3):327–333. doi: 10.1016/0378-1097(94)90524-x. [DOI] [PubMed] [Google Scholar]
  6. Legisa M., Gradisnik-Grapulin M. Sudden substrate dilution induces a higher rate of citric acid production by Aspergillus niger. Appl Environ Microbiol. 1995 Jul;61(7):2732–2737. doi: 10.1128/aem.61.7.2732-2737.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Maresca B., Medoff G., Schlessinger D., Kobayashi G. S. Regulation of dimorphism in the pathogenic fungus Histoplasma capsulatum. Nature. 1977 Mar 31;266(5601):447–448. doi: 10.1038/266447a0. [DOI] [PubMed] [Google Scholar]
  8. Pall M. L. Adenosine 3',5'-phosphate in fungi. Microbiol Rev. 1981 Sep;45(3):462–480. doi: 10.1128/mr.45.3.462-480.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Pall M. L. Cyclic AMP and the plasma membrane potential in Neurospora crassa. J Biol Chem. 1977 Oct 25;252(20):7146–7150. [PubMed] [Google Scholar]
  10. Paveto C., Passeron S. Some kinetic properties of Mucor rouxii phosphofructokinase. Effect of cyclic adenosine 3', 5'-monophosphate. Arch Biochem Biophys. 1977 Jan 15;178(1):1–7. doi: 10.1016/0003-9861(77)90164-3. [DOI] [PubMed] [Google Scholar]
  11. Thevelein J. M., Beullens M., Honshoven F., Hoebeeck G., Detremerie K., den Hollander J. A., Jans A. W. Regulation of the cAMP level in the yeast Saccharomyces cerevisiae: intracellular pH and the effect of membrane depolarizing compounds. J Gen Microbiol. 1987 Aug;133(8):2191–2196. doi: 10.1099/00221287-133-8-2191. [DOI] [PubMed] [Google Scholar]
  12. Thevelein J. M. Fermentable sugars and intracellular acidification as specific activators of the RAS-adenylate cyclase signalling pathway in yeast: the relationship to nutrient-induced cell cycle control. Mol Microbiol. 1991 Jun;5(6):1301–1307. doi: 10.1111/j.1365-2958.1991.tb00776.x. [DOI] [PubMed] [Google Scholar]
  13. Thevelein J. M. Signal transduction in yeast. Yeast. 1994 Dec;10(13):1753–1790. doi: 10.1002/yea.320101308. [DOI] [PubMed] [Google Scholar]
  14. Trevillyan J. M., Pall M. L. Control of cyclic adenosine 3',5'-monophosphate levels by depolarizing agents in fungi. J Bacteriol. 1979 May;138(2):397–403. doi: 10.1128/jb.138.2.397-403.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Wold W. S., Suzuki I. Cyclic AMP and citric acid accumulation by Aspergillus niger. Biochem Biophys Res Commun. 1973 Jan 23;50(2):237–244. doi: 10.1016/0006-291x(73)90831-0. [DOI] [PubMed] [Google Scholar]

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