Figure 3.

Effects of PMTS on α2 (S270C)β1 and α2 (S291C)β1 GABA_A receptors expressed in Xenopus oocytes. (A) In α2 (S270C)β1 GABA_A receptors, PMTS (50 μM) irreversibly enhanced the GABA response. Washout from PMTS was measured every 10 min for a total of 30 min. (B) In α2 (S291C)β1 GABA_A receptors, PMTS did not affect the GABA response. Isoflurane (0.6 mM) potentiated α2 (S291C)β1 GABA receptor function equally before and after application of PMTS. PMTS or isoflurane were perfused for 60 s before being coapplied with GABA for 30 s. Data are the mean ± SEM of five to six oocytes.