Figure 4.

Effects of anesthetics on α2β1 or α2(S270C)β1 GABA_A receptors, expressed in Xenopus oocytes, before and after perfusion with PMTS. (A) In wild-type α2β1 receptors, enflurane (1.2 mM), isoflurane (0.6 mM), octanol (0.1 mM), or alphaxalone (1 μM) enhanced GABA responses before and after application of PMTS (50 μM). (B) In mutant α2(S270C)β1 receptors, application of PMTS abolished the effects of enflurane, isoflurane, or octanol but did not affect the enhancement by alphaxalone. PMTS was perfused for 60 s before being coapplied with GABA for 30 s. Anesthetics or octanol were tested again after 15 min of washout from PMTS. Data are expressed as a mean ± SEM of five to 12 oocytes.