Figure 2.

Localization of IL-6 receptor in pituitary and adrenal. (A) IL-6 receptor colocalizes with ACTH to pituitary corticotrophs. Pituitaries from WT mice were stained with immunofluorescently labeled antibodies. Staining specific for ACTH (green) is shown (Left) or IL-6 receptor (IL-6R) (red) is shown (Middle). Colocalization of both ACTH and IL-6 receptor is indicated by yellow cells (Right). (B) IL-6 receptor is located in mouse adrenal cortex. (Left) A hematoxylin/eosin stained section of WT mouse adrenal is shown. (Right) Autoradiogram of an adjacent adrenal section subjected to in situ hybridization with a probe for mouse IL-6 receptor is shown. M, medulla; ZF, zona fasciculata.