Skip to main content
. 2000 Jul 18;97(16):9323–9328. doi: 10.1073/pnas.150005697

Figure 1.

Figure 1

Summary of positional cloning experiments leading to isolation of the DND1 gene. (A) CHS1, pCIT1243, and nga106 are previously mapped molecular genetic markers on Arabidopsis chromosome 5. pCIT1243 and nga106 were used to identify BAC clones carrying wild-type Col-0 genomic DNA inserts. Inserts from four BACs were subcloned to create a binary cosmid library; some of these cosmids were used as additional restriction fragment length polymorphism markers to refine the genetic interval containing DND1. Three cosmids that complement the dnd1 mutation were identified of 43 tested in a focused shotgun complementation project. (B) Higher-resolution complementation experiments using more precise subclones identified additional complementing and noncomplementing clones, including other clones not shown, that implicated an ≈5-kb region as the DND1 locus. This region encodes a previously identified cDNA named AtCNGC2. Cosmids 1H3 and 2A1 are shown in both A and B. E, EcoRI site; X, XbaI site.