Figure 3.

Complementation of the reduced-HR and constitutive resistance phenotypes of dnd1 plants by transformation with cloned wild-type DND1. (A) HR scores for leaves inoculated with P. syringae pv. glycinea Race 4 carrying cloned avrRpt2 (filled bars) or an empty plasmid vector (open bars). HR tissue collapse scored on a scale of 0–5 (0 = no visible collapse; 5 = confluent collapse of inoculated tissue; values are mean ± SE). Data are shown for T₂ plants derived from hemizygous primary transformants; 1A8 (dwarf) and 1H2 (dwarf) represent control sets of T₂ segregants that returned to the dnd1/dnd1 dwarf rosette size because of loss (via segregation) of the DND1 transgene. (B) Growth of virulent P. syringae pv. tomato DC3000 in leaf tissue of inoculated plants. Col-0, wild type; 1H3, Col-0 dnd1/dnd1 line carrying wild-type DND1 because of transformation with cosmid 1H3; dnd1, Col-0 dnd1/dnd1; 1H3 (dwarf), dwarf-sized T₂ plants from a Col-0 dnd1/dnd1 T₁ transformant that was hemizygous for the introduced 1H3 construct.