Figure 2.

Chromatin immunoprecipitation (ChIP) analyses of vernalization-mediated histone modifications in wild type and vil1. Samples are from plants grown as follows: nonvernalized (NV); vernalized for 40 d (40V); vernalized for 40 d and subsequently grown for 14 d at 22°C (40VT14). Relative fold changes are indicated at the bottom of each ChIP assay. All assays were repeated with at least two independent chromatin preparations and three independent immunoprecipitations as described in Materials and Methods. Representative ChIP results are shown using antibodies recognizing acetylated Histone H3 (A), dimethylated Histone H3 Lys 9 (B), dimethylated Histone H3 Lys 27 (C), trimethylated Histone H3 Lys 9 (D), and trimethylated Histone H3 Lys 27 (E). The regions of FLC and FLM chromatin examined are described in Supplementary Figure S2.