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. 2006 Dec 1;20(23):3244–3248. doi: 10.1101/gad.1493306

Figure 4.

Figure 4.

(A) Effect of vernalization on mRNA levels of FLC and FLM in wild-type (Col) and vil1 mutants grown in SD. Samples in all panels unless noted are nonvernalized (−) or cold-treated for 40 d and subsequently grown for 10 d at 22°C under SD (+). (Note: In Scortecci et al. [2001], we reported that FLM mRNA levels were not reduced by vernalization in a FRI-containing line. However, we now reproducibly detect a vernalization-mediated decrease of FLM with or without FRI.) (B) ChIP assays using antibodies recognizing trimethylated Histone H3 Lys 9 at FLM chromatin in wild type (Col), vin3, and vil1. (C) ChIP assay using antibodies recognizing trimethylated Histone H3 Lys 27 at FLM chromatin in wild type (Col), vin3, and vil1. (D) ChIP assay using antibodies recognizing hyperacetylated Histone H4 at FLM chromatin in vil1 mutants. (E) mRNA expression patterns of floral integrator genes in wild-type (Col), flm, and flc mutants grown in SD. cDNA templates prepared from mutant seedlings were intentionally diluted relative to wild-type samples to emphasize the increased mRNA levels in mutants. (F) Relationship of VIN3 family genes to the regulatory network controlling flowering time in response to environmental cues.