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. 2006 Dec 1;20(23):3311–3323. doi: 10.1101/gad.1466806

Figure 2.

Figure 2.

Neurofibromin is expressed in post-mitotic neurons of the larval CNS. (A–F) Confocal images of anti-NF1-stained wild-type (A,C,E) or NF1E2 (B,D,F) first (A,B), second (C,D), or third (E,F) instar CNS. Bars: A,C, 100 μm; E, 200 μm. (G) Lack of overlap between NF1 (red) and BrdU (green) staining. (H,I) Confocal images of the central brain and ventral ganglion regions indicated by hatched boxes in E. (Red) NF1; (green) phalloidin. No obvious differences in staining intensity or in subcellular localization were observed when comparing wild-type and NF1E4 mutants (not shown). (J–L) Lack of overlap between anti-NF1 (red) and repo-GAL4-driven UAS-GFP (green) staining. The region imaged is part of the third instar central brain. (M–O) No overlap between anti-NF1 (red) and grh-lacZ (green) expression in neuroblasts. (P–R) NF1 staining (red) is not obvious in third instar MBs, identified by UAS-GFP expression directed by the 201Y MB GAL4 driver.