Figure 4.

Large segments of neurofibromin other than the GRD are dispensable for size rescue. (A) Extent of four in-frame deletions. (B) IP-Western analysis of adult head extracts showing different levels of heat-shock-inducible (hs) transgene expression. (C) Female pupal size of flies of the indicated genotypes, which did (filled-in columns) or did not (open columns) receive daily heat shocks. The graph shows the average female pupal length and the standard deviation calculated by measuring >20 pupae for each genotype. Asterisks indicate significant rescue (Student t-test, p < 0.0001).