Figure 4.

Differential expression of a gene-specific sequence (D251) in V. faba plants. Total RNA was isolated from stem or leaf tissue of plants exposed to air, methyl jasmonate, or (Z)-jasmone. Ten micrograms RNA per sample was loaded and separated on a formaldehyde gel (A). The samples then were transferred to a nylon membrane and probed with the D251 sequence (B). Lane 1 = air treatment (control); lane 2 = (Z)-jasmone treatment; lane 3 = methyl jasmonate treatment. In the case of RNA isolated from leaf tissue, only (Z)-jasmone treatment results in up-regulation.