Figure 2.

(A) Two representative tethering experiments. The protein TS is present at a concentration of 15 μM, and each of the 10 disulfide library members in each pool is present at 200 μM. The buffer contains 25 mM potassium phosphate (pH 7.5) and 1 mM 2-mercaptoethanol, and the samples were allowed to equilibrate at ambient temperature for 1 h before analysis. (B) Three tethering experiments in which the concentrations of 2-mercaptoethanol were varied as stated. The pool of disulfides is the same as in A Right, and the conditions (other than 2-mercaptoethanol concentration) were the same as above. (C) Three tethering experiments in which the pool size and concentrations were varied as stated. All other conditions were the same as above.