Detection by immunofluorescence of PfCRT expression in HEK293 cells. Bright field (A, C, E, G) and epifluorescence (B, D, F, H) images of HEK293 cells, either mock transfected (A, B, E, F) or transfected with Dd2 (C, D) or HB3-myc (G, H). Cells were fixed in paraformaldehyde and permeabilized with Triton X-100. In panels B and D, mock-transfected (B) and Dd2-transfected (D) cells were probed with anti-PfCRT primary antibodies. In panels F and H, mock-transfected (F) and HB3-myc-transfected (H) cells were probed with anti-myc primary antibodies. Scale bar = 10 μm.