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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Mol Biochem Parasitol. 2006 Sep 25;150(2):288–299. doi: 10.1016/j.molbiopara.2006.09.001

Fig. 2.

Fig. 2

Colocalization of PfCRT and LAMP-2. Bright field (A, E, I) and epifluorescence images (B-D, F-H, J-L) of doubly labeled, PfCRT-expressing HEK293 cells, probed with rabbit anti-PfCRT antibodies detected via Cy3-secondary anti rabbit IgG antibodies (B, F, J; red), as well as mouse monoclonal antibodies against LAMP-2 (C), calnexin (G) or GM-130 (K), detected with Alexa Fluor 488-labeled secondary anti-mouse IgG antibodies (green). (D, H, L) Overlays of the red and green channels. Yellow indicates areas of overlap. Scale bar = 10 μm.