Skip to main content
. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Mol Biochem Parasitol. 2006 Sep 25;150(2):288–299. doi: 10.1016/j.molbiopara.2006.09.001

Fig. 4.

Fig. 4

Subcellular fractionation of HEK293 cells expressing PfCRT. Western blot of transfected HEK293 cell fractions. Cells were mock-transfected (water), or transfected with HB3-myc or HB3-NTD-myc. The blot was probed with anti-myc tag antibodies, and developed via ECL. Key to fractions: P1 = 800 g pellet, P2 = 14,000 g pellet, P3 = 100,000 g pellet. Note that, as expected, the molecular weight of the N-terminal domain deletion (HB3-NTDmyc) construct is about 5 kDa less than full length PfCRT (HB3-myc).