Subcellular fractionation of HEK293 cells expressing PfCRT. Western blot of transfected HEK293 cell fractions. Cells were mock-transfected (water), or transfected with HB3-myc or HB3-NTD-myc. The blot was probed with anti-myc tag antibodies, and developed via ECL. Key to fractions: P1 = 800 g pellet, P2 = 14,000 g pellet, P3 = 100,000 g pellet. Note that, as expected, the molecular weight of the N-terminal domain deletion (HB3-NTDmyc) construct is about 5 kDa less than full length PfCRT (HB3-myc).