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. Author manuscript; available in PMC: 2007 Dec 1.
Published in final edited form as: Mol Biochem Parasitol. 2006 Sep 25;150(2):288–299. doi: 10.1016/j.molbiopara.2006.09.001

Fig. 6.

Fig. 6

GPN-induced lysosomal Ca2+ release monitored by Fura-2 imaging. Cells were loaded with Fura-2-AM and the cytoplasmic Ca2+ concentration was determined continuously by ratiometric imaging. (A) Mean cytoplasmic Ca2+ of seven HEK293 cells in a field during application of GPN (200 μM) and carbachol (100 μM). (B) Mean peak lysosomal Ca2+ release by GPN from HEK293 cells transfected with RFP controls, HB3-RFP or Dd2-RFP. The means are not significantly different. Error bars indicate S.E.M.