Table 3.
Investigation Title | University of Heidelberg H sapiens TK6 | ||
Experimental Designs | genetic_modification_design | time_series_design | |
Experimental Factors | GeneticModification | Time | |
Person Last Name | Maier | Fleckenstein | Li |
Person First Name | Patrick | Katharina | Li |
Person Email | patrick.maier@radonk.ma.uni-heidelberg.de | ||
Person Phone | +496213833773 | ||
Person Address | Theodor-Kutzer-Ufer 1–3 | ||
Person Affiliation | Department of Radiation Oncology, University of Heidelberg | ||
Person Roles | submitter; investigator | investigator | investigator |
Quality Control Types | biological_replicate | ||
Replicate Types | biological_replicate | ||
Date of Experiment | 2005-02-28 | ||
Public Release Date | 2006-01-03 | ||
PubMed ID | 12345678 | ||
Publication Author List | Patrick Maier; Katharina Fleckenstein; Li Li; Stephanie Laufs; Jens Zeller; Stefan Fruehauf; Carsten Herskind; Frederik Wenz | ||
Publication Status | submitted | ||
Experiment Description | Gene expression of TK6 cells transduced with an oncoretrovirus expressing MDR1 (TK6MDR1) was compared to untransduced TK6 cells and to TK6 cell transduced with an oncoretrovirus expressing the Neomycin resistance gene (TK6neo). Two biological replicates of each were generated and the expression profiles were determined using Affymetrix Human Genome U133 Plus2.0 GeneChip microarrays. Comparisons between the sample groups allow the identification of genes with expression dependent on the MDR1 overexpression. | ||
Protocol Name | GROWTHPRTCL10653 | EXTPRTCL10654 | TRANPRTCL10656 |
Protocol Type | grow | nucleic_acid_extraction | bioassay_data_transformation |
Protocol Description | TK6 cells were grown in suspension cultures in RPMI 1640 medium supplemented with 10% horse serum (Invitrogen, Karlsruhe, Germany). The cells were routinely maintained at 37 C and 5% CO2. | Approximately 10 cells were lysed in RLT buffer (Qiagen).Total RNA was extracted from the cell lysate using an RNeasy kit (Qiagen). | Mixed Model Normalization with SAS Micro Array Solutions (version 1.3). |
Protocol Parameters | media | Extracted Product; Amplification | |
SDRF Files | e-mexp-428_tab.txt | ||
Database | CTO | MO | nci_meta |
Database URI | http://obo.sourceforge.net/cgi-bin/detail.cgi?cell | http://mged.sourceforge.net/ontologies/MGEDontology.php | http://ncimeta.nci.nih.gov/indexMetaphrase.html |
Database Version | 1.3.0.1 |
The first column represents the qualifier name, while their values are given starting from column 2 (if the qualifier has two or more values, each is given in a separate column).