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. 2006 Nov 6;7:489. doi: 10.1186/1471-2105-7-489

Table 3.

An example of an IDF.

Investigation Title University of Heidelberg H sapiens TK6
Experimental Designs genetic_modification_design time_series_design
Experimental Factors GeneticModification Time
Person Last Name Maier Fleckenstein Li
Person First Name Patrick Katharina Li
Person Email patrick.maier@radonk.ma.uni-heidelberg.de
Person Phone +496213833773
Person Address Theodor-Kutzer-Ufer 1–3
Person Affiliation Department of Radiation Oncology, University of Heidelberg
Person Roles submitter; investigator investigator investigator
Quality Control Types biological_replicate
Replicate Types biological_replicate
Date of Experiment 2005-02-28
Public Release Date 2006-01-03
PubMed ID 12345678
Publication Author List Patrick Maier; Katharina Fleckenstein; Li Li; Stephanie Laufs; Jens Zeller; Stefan Fruehauf; Carsten Herskind; Frederik Wenz
Publication Status submitted
Experiment Description Gene expression of TK6 cells transduced with an oncoretrovirus expressing MDR1 (TK6MDR1) was compared to untransduced TK6 cells and to TK6 cell transduced with an oncoretrovirus expressing the Neomycin resistance gene (TK6neo). Two biological replicates of each were generated and the expression profiles were determined using Affymetrix Human Genome U133 Plus2.0 GeneChip microarrays. Comparisons between the sample groups allow the identification of genes with expression dependent on the MDR1 overexpression.
Protocol Name GROWTHPRTCL10653 EXTPRTCL10654 TRANPRTCL10656
Protocol Type grow nucleic_acid_extraction bioassay_data_transformation
Protocol Description TK6 cells were grown in suspension cultures in RPMI 1640 medium supplemented with 10% horse serum (Invitrogen, Karlsruhe, Germany). The cells were routinely maintained at 37 C and 5% CO2. Approximately 10 cells were lysed in RLT buffer (Qiagen).Total RNA was extracted from the cell lysate using an RNeasy kit (Qiagen). Mixed Model Normalization with SAS Micro Array Solutions (version 1.3).
Protocol Parameters media Extracted Product; Amplification
SDRF Files e-mexp-428_tab.txt
Database CTO MO nci_meta
Database URI http://obo.sourceforge.net/cgi-bin/detail.cgi?cell http://mged.sourceforge.net/ontologies/MGEDontology.php http://ncimeta.nci.nih.gov/indexMetaphrase.html
Database Version 1.3.0.1

The first column represents the qualifier name, while their values are given starting from column 2 (if the qualifier has two or more values, each is given in a separate column).