Figure 1.

(A and B) Laminar shear stress increases the level of p53 in endothelial cells. In A, BAECs were subjected to laminar shear stress of 0, 1.5, 3, 6, and 12 dynes/cm² for 24 h followed by immunoblotting by using an anti-p53 mAb. In B, BAECs were subjected to laminar shear stress at 12 dynes/cm² for periods of time as indicated, followed by immunoblotting by using an anti-p53 mAb. (C) Laminar shear stress of 12 dynes/cm² increases p53 phosphorylation by JNK. BAECs were subjected to laminar shear stress at 12 dynes/cm² for periods of time as indicated or kept as static control represented by time 0. JNK was immunoprecipitated from BAEC lysates with an anti-JNK1 antibody, and the kinase activity of the isolated JNK was tested by using GST-p53 as a substrate in the presence of (γ-³²P)ATP.