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. 2000 Aug 8;97(17):9396–9401. doi: 10.1073/pnas.170283697

Figure 2.

Figure 2

The imino spectra of H3–18 at different temperatures (0.1 mM RNA concentration, 50 mM NaCl, pH 6.5, 1,024 scans). The melting of the G8, G11, and U12 imino peaks occurs near the same temperature as the minor UV melting transition (48°C), whereas the G imino protons from the stem still show sharp peaks. The stem imino peaks G4 and G5 shift significantly with the transition from dimer to monomer, but G2, U3, and G6 do not. Schematics of a kissing dimer (20°C) and melted monomers (48°C) are shown.