|
Number of NOE distance restraints |
|
Total number of the NOEs |
528 |
Intramolecular-Intranucleotide NOEs |
182 |
Intramolecular-Internucleotide NOEs |
298 |
Intermolecular NOEs |
32 |
Kissing loop with closing base pairs |
170 |
Negative NOEs |
16 |
Dihedral angle restraints (δ, χ and ɛ for all residues, α and ζ for C10) |
194 |
A-form geometry dihedral restraints (α,β,γ,ζ) for the stems except for closing bps |
98 |
Base planarity restraints |
32 |
Total number of restraints |
852 |
rms deviation for all heavy atoms relative to the average structure, Å |
The kissing loop with closing base pairs (A7–U12, A7′–U12′) |
1.1 ± 0.2 |
Stem 1 (G1–G6, C13–C18) |
1.5 ± 0.5 |
Stem 2 (G1′–G6′, C13′–C18′) |
1.2 ± 0.6 |
NOE violations, Å |
0 (> 0.2 Å) |
Angle violations, ° |
1 (> 5°) |
Mean deviation from covalent geometry |
Bond length, Å |
0.004 |
Angles, ° |
0.81 |
Impropers, ° |
0.16 |