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YpkA overexpression causes actin filament disruption. HeLa cells were cotransfected with a vector encoding the enhanced green fluorescent protein (250 ng) and either YpkA, YpkA-K269A, YpkA-Δ450, YpkA-Δ660, YpkA-Δ710, or pCDNA3 (1.75 μg). Cell morphology was visualized by expression of enhanced green fluorescent protein. Actin filaments were visualized by staining with rhodamine phalloidin. Pictures shown are representative of all transfected cells stained.
