Figure 2.

In vitro translation analysis of the prostate-specific TCRγ transcript. The prostate-specific TCRγ transcript encodes two proteins in vitro. [³⁵S]Met-labeled in vitro-translated proteins were run on a Tris/Tricine-buffered 16.5% polyacrylamide gel and analyzed by autoradiography. A schematic representation of the mutant constructs used is shown on the right. An open box represents the first reading frame with potential initiation codons in bold, whereas the second reading frame is represented by a shaded box with the potential initiation codon in italics. “X” indicates an ATG codon mutated to ATA. Size markers in kDa are indicated on the top.