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. 2000 Aug 8;97(17):9437–9442. doi: 10.1073/pnas.160270597

Figure 3.

Figure 3

TARP is nuclear protein expressed in prostate extracts. (A) Western blot of protein extracts derived from LNCaP cells, PC3 cells, or a prostate tumor sample (Cancer). Each protein extract (20 μg) was run on a Tris/Tricine 16.5% polyacrylamide gel and probed with an antibody against TARP (Upper) or TCRγ (Lower). As a positive control, 1 μg of His-tagged TARP (Upper) or 100 ng of His-tagged TCRγ (Lower) was run on the gel (Recomb.). Size markers in kDa are indicated on the left. (B) Western blot of the cytoplasmic fraction (Cytoplasm), membrane fraction (Membrane), and nuclear fraction (Nucleus) of LNCaP cells. Each fraction (40 μg) was run on a Tris/Tricine 16.5% polyacrylamide gel and probed with an antibody against TARP. As a positive control, 1 μg of His-tagged TARP was run on the gel (Recomb.). Size markers in kDa are indicated on the left.