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TARP exists in the nuclei of breast cancer cells. Western blot of nuclear extracts derived from LNCaP, MCF7, BT-474, SK-BR-3, and Hs57BsT cells. Each nuclear extract (40 μg) was run on a Tris/Tricine 16.5% polyacrylamide gel and probed with an antibody against TARP (Upper) or TCRγ (Lower). As a positive control, 1 μg of His-tagged TARP (His-TARP) and 100 ng of His-tagged TCRγ (His-TCRγ) were run on the gels. Size markers in kDa are indicated on the left.
