Figure 2.

The transactivation domain of Nkx2.2 maps to the C terminus. A reporter plasmid containing five tandem copies of the GAL4 UAS upstream of the E1b minimal promoter driving luciferase and an expression plasmid encoding each GAL4DBD-Nkx2.2 fusion construct were cotransfected with a CMV promoter-driven β-galactosidase expression plasmid. All luciferase activities are corrected for β-galactosidase activity. Relative luciferase activities are calculated, with the activity of cells transfected with the GAL4DBD alone set at 1. All data are shown as mean ± SEM. (A) The reporter plasmid is indicated schematically. (B) The results of the one-hybrid assays. (Left) Results from βTC3 cells. (Right) Results from NIH 3T3 cells. (C) Results of detailed mapping of the activation domain. (Left) Results from βTC3 cells. (Right) Results from NIH 3T3 cells.