Figure 3.

Full-length Nkx2.2 acts as a weak repressor in βTC3 cells. A reporter plasmid containing five tandem copies of the GAL4 UAS upstream of the prolactin minimal promoter driving luciferase and an expression plasmid encoding each GAL4DBD-Nkx2.2 fusion construct or a GAL4DBD-Pax6 activation domain fusion construct were cotransfected with CMVβgal. All luciferase activities are corrected for β-galactosidase activity. Relative luciferase activities are calculated, with the activity of cells transfected with the GAL4DBD alone set at 1. All data are shown as mean ± SEM.