Table 1.

Apparent dissociation constants for binding to ()Nkx2.2

Oligonucleotide*	Sequence†	Relative binding affinity‡
Nkx2.2 consensus	TTTTTAAGTGGTTTTT	1.0
M1	TTTTCAAGTGGTTTTT	5.2  ± 1.0
M2	GGGTTAAGTGGGGGGG	0.55  ± 0.17
M3	TTTTTACGTGGTTTTT	0.067  ± 0.003
M4	TTTTTAAGTGGTTGGG	0.33  ± 0.02
M5	GGGTTAAGTGGTTTTT	0.70  ± 0.19
M6	TTTTTAAGTGGGGTTT	0.45  ± 0.02

^*Designation for the individual oligonucleotides are the same as those in Fig. 2. 

^† Alignment with the Nkx2.2 consensus sequence is shown. Changes from the consensus are underlined. The complete sequences of the oligonucleotides are provided in Materials and Methods. 

^‡ Affinities of the individual oligonucleotides for ()Nkx2.2 were determined by competition EMSA, followed by PhosphorImager analysis, as detailed in Materials and Methods. The apparent dissociation constant (K_d) for the consensus oligonucleotide is 3.12 × 10⁻⁸ M; the relative affinities of the other oligonucleotides are expressed as a decimal fraction of this value. Data represent a mean ± SEM of at least three separate determinations.