Figure 5.

Genomic organization of the desat locus in CS strain. Two hybridization-positive EcoRI fragments were obtained after hybridization of a CS genomic library with desat1 probe. The number of * show the intensity of the hybridization signal with the desat1 probe. EcoRI and HindIII restriction sites are noted E and H, respectively. Putative desat2 ORF and desat1 complete cDNA are in bold. The intron sizes of desat1 and putative desat2 genes are noted under their representations. Primers used for RT-PCR are indicated by small arrows. Dotted line indicates the position of the putative desat1 promoter.