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(A) Stereospecificity of hydroxylation of 4-ethylphenol by wild-type VAO and Asp-170/Thr-457 mutants in 50 mM potassium phosphate buffer (pH 7.5) at 25°C. (R)-1-(4′-hydroxyphenyl)ethanol (black) and (S)-1-(4′-hydroxyphenyl)ethanol (white). (B) Expanded chiral GC separation pattern of the hydroxylation of 4-ethylphenol by (I) wild-type VAO and (II) D170S/T457E.
