Table 1.

Kinetic parameters of wild type VAO and Asp-170/Thr-457 mutants with 4-(methoxymethyl)phenol in air-saturated 50 mM potassium phosphate buffer (pH 7.5) at 25°C

Parameter	Wild-type*	D170S†	D170A†	T457E	D170S/T457E	D170A/T457E
kcat (s−1)	3.1	0.004	0.001	1.3	0.05	0.02
Km (μM)	55	ND	ND	168	238	65
kred (s−1)‡	3.3	0.005	0.002	1.2	0.03	0.02

ND, not determined. 

^*Data from ref. 7. 

^† Data from ref. 11. 

^‡ k_red was measured at a substrate concentration of 500 μM.