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. 2000 Aug 1;97(17):9498–9503. doi: 10.1073/pnas.150006697

Figure 3.

Figure 3

p21CIP1 levels are diminished in cells overexpressing c-Myc. NIH 3T3 cells were plated at 1.5 × 106 cells/100-mm dish (A) or 7 × 105 cells/100-mm dish (B) and transiently transfected 24 hr later with either empty CMV vector or CMV-Myc. PolyA+ mRNA was isolated, and approximately 1 μg RNA per sample was analyzed by Northern blot analysis. The blot was sequentially probed for both p21CIP1 (Top, Upper) and cyclophilin (Top, Lower). The Northern blot was then analyzed by densitometry, and relative levels of p21CIP1 mRNA normalized by levels of cyclophilin were plotted on a bar graph, where p21CIP1 levels in vector-only-transfected cells were set to a value of one for comparison with c-Myc transfected cells (Bottom).