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. 2000 Aug 15;97(17):9525–9530. doi: 10.1073/pnas.97.17.9525

Figure 1.

Figure 1

Targeting the capsulin locus. (A) The structure of the capsulin gene and surrounding genomic region is shown at the top, the lacZ targeting vector is in the middle, and the targeted allele is at the bottom. Noncoding region is in white, coding region in black, and the bHLH region in gray. The targeting vector introduced lacZ in-frame with the initiation codon of capsulin and a neomycin-resistance gene immediately downstream. The thymidine kinase gene at the 3′ end of the targeting vector was used for negative selection. The targeting strategy resulted in deletion of the entire first exon, which encodes the bHLH region. The position of the external probe used for Southern analysis in B is indicated. Genotyping of ES cells and offspring was performed by SacI digestion of DNA and hybridization to the indicated probe or by PCR. Arrows designate the positions of PCR primers used for genotyping. (B) Southern blot of genomic DNA from wild-type (+/+) and capsulin+/− mice. The probe shown in A, which is external to the region of vector homology, detects a wild-type band of 6.0 kb and a mutant band of 3.5 kb, after digestion with SacI.