Figure 6.

Apoptosis in the splenic lineage of capsulin mutant embryos revealed by TUNEL staining. Histologic cross sections through abdominal sections of wild-type and capsulin mutant embryos at E12.5 were stained by TUNEL staining to detect apoptosis. Center and Right show the dorsal and ventral regions, respectively, of the developing spleen with extensive apoptosis (yellow) localized to the splenic primordium. p, pancreas; s, spleen; st, stomach. In the wild-type embryo (Left), there is only an occasional TUNEL-positive cell with no preferential localization to any particular tissue. The wild-type section corresponds to an expanded region of the section in Fig. 5B, and the mutant sections correspond to expanded regions of the section in Fig. 5C.