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. 2003 Jul 1;31(13):3758–3762. doi: 10.1093/nar/gkg580

Figure 1.

Figure 1

Figure 1

(A) Hybridization of the C.elegans cytochrome P450 LNA oligonucleotide microarray with a pool of 10 biotin-labelled antisense oligonucleotides. The 50 gene-specific CYP450 50mer LNA oligonucleotides were spotted in quadruplicate along with Cy3 labelled marker oligonucleotides (enclosed in white boxes) onto the Immobilizer microarray slide (Exiqon, Denmark). The resulting microarray was hybridized with a pool of 10 5′-biotin-labelled 50mer antisense target oligonucleotides at 65°C in 0.5 M NaCl, followed by staining with Streptavidin-Cy3 and scanning in a confocal laser scanner (ScanArray Express HT, Perkin Elmer, USA). A specific hybridization pattern is obtained from the 10 CYP450 LNA capture probes, whereas no cross-hybridization is observed for the remaining 40 CYP450 oligonucleotides. (B) Assessment of the specificity of the C.elegans CYP450 LNA oligonucleotide capture probes. Processing of the microarray data (GenePix Pro 4.01, Axon, USA) revealed strong capture probe-specific hybridization signals for the 10 CYP450 LNA oligonucleotides with a several fold higher signal intensity compared to the probe showing highest cross-hybridization.