Figure 1.

[³⁵S]DmAMP1 binding capacity of wild-type yeast, DM1 cells, and DM1 cells transformed with pRS423, pRS423(IPT1), pYX233, and pYX233(IPT1). [³⁵S]DmAMP1 (40 nM) was incubated with wild-type yeast cells (a), DM1 cells (b), or DM1 cells transformed with pRS423 (c), pRS423(IPT1) (d), pYX233 (e), or pYX233(IPT1) (f). Binding assays were performed after 1 h of incubation at 22°C. The growth medium was medium A for all strains, except for DM1 cells transformed with pYX233 or pYX233(IPT1), for which the galactose-containing medium B was used (see Materials and Methods for medium composition). Data are means ± SE of triplicate measurements and correspond to one representative experiment of two.