Figure 3.

Membrane permeabilization induced by DmAMP1 on wild-type yeast, DM1 cells, and DM1 cells transformed with either pYX233 or pYX233(IPT1). Dose–response curves are presented for membrane permeabilization measured by SYTOX green fluorescence of wild-type yeast (■), DM1 cells (×), and DM1 cells transformed with either pYX233 (▴) or pYX233(IPT1) (●). Wild-type yeast and DM1 cells were suspended in medium A supplemented with 5 mM MgCl₂ and 0.2 μM SYTOX green. DM1 cells transformed with either pYX233 or pYX233(IPT1) were suspended in medium B supplemented with 5 mM MgCl₂ and 0.2 μM SYTOX green. Yeast cells were incubated in the presence of DmAMP1 for 12 h, after which fluorescence was measured. Data are means ± SE of triplicate measurements and correspond to one representative experiment of two.