Figure 4.

DmAMP1-resistant yeast strains lack the sphingolipid M(IP)₂C. Preparation of [³H]inositol-labeled cells, lipid extraction, TLC, tritium detection, and location of internal sphingolipid standards are described in Materials and Methods. The location of internal standards is indicated below the peaks, as is the location of glycerol phosphoinositol (GPI). Strains DM2 and DM3 gave a TLC profile identical to that of strain DM1 (data not shown). Differences in migration distances are due to lane-to-lane variation in mobility.