Table 1.

Antifungal activity of DmAMP1 against various S. cerevisiae strains

S. cerevisiae strain	IC50, μM*	Medium†
YPH250	1.2  ± 0.2	A
RCD113 ipt1-deletion strain	> 40	A, B
W303-1A wild type (haploid)	0.7  ± 0.1	A
W303 wild type (diploid)	1.0  ± 0.2	A
DM1, DM2, and DM3 DmAMP1-resistant strains	> 40	A
W303-1A × DM1 (diploid)	1.7  ± 0.2	A
DM4 DmAMP1-resistant strain	4.5  ± 0.6	A
DM1 transformed with pRS423	> 40	A
DM1 transformed with pRS423(IPT1)	1.1  ± 0.2	A
DM1 transformed with pYX233	> 40	B
DM1 transformed with pYX233(IPT1)	1.1  ± 0.2	B
RCD113 transformed with pYX233(IPT1)	1.2  ± 0.2	B
W303-1AΔsur1	1.4  ± 0.2	A
DM1Δsur1	8.7  ± 0.7	A

^*IC₅₀ values (i.e., the concentration of the antifungal protein that is required to inhibit 50% of the growth) are expressed as mean ± SE (n = 3). 

^† Media used to grow the yeast strains were either medium A or medium B (see Materials and Methods for composition).